| 质粒名称: | pEGFP-C1-WEEV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002850 |
| 产品用途: | Mammalian expression of mEGFP-WEEV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
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载体骨架基本信息 |
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| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
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插入基因信息 |
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| 插入基因名称: | WEEV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 大肠杆菌表达优化 |
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文献引用方法 |
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| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
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pEGFP-C1-WEEV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEGFP-C1-WEEV nsP3载体质粒基本信息
pEGFP-C1-WEEV nsP3质粒图谱载体图谱和pEGFP-C1-WEEV nsP3载体序列质粒序列多克隆位点信息
pEGFP-C1-WEEV nsP3质粒载体简介
pEGFP-C1-WEEV nsP3质粒序列载体序列
LOCUS pEGFP-C1-WEEV nsP3 6277 bp DNA circular SYN 16-JUL-2026 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP-C1-WEEV nsP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6277) AUTHORS 1243235 TITLE Direct Submission JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..6277 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 304..507 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 552..1268 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1274..1298) /label=p61-1155 primer_bind 1284..1322 /label=p61-1150 CDS 1299..2876 /codon_start=1 /label=WEEV nsP3 /translation="APAYRVIRGDISKSADQAIVNAANSKGQPGSGVCGALYRKWPAAF DRQPIAVGTARLVKHEPLIIHAVGPNFSKMPEPEGDLKLAAAYMSIASIVNAERITKIS VPLLSTGIYSGGKDRVMQSLHHLFTAFDTTDADVTIYCLDKQWETRIIEAIHRKESVEI LDDDKPVDIDLVRVHPNSSLAGRPGYSVNEGKLYSYLEGTRFHQTAKDIAEIHAMWPNK SEANEQICLYILGESMSSIRSKCPVEESEASAPPHTLPCLCNYAMTAERVYRLRSAKKE QFAVCSSFLLPKYRITGVQKLQCSKPVLFSGVVPPAVHPRKYAEIILETPPPPATTTVI CEPTVPERIPSPVISRAPSAESLLSLGGVSFSSSATRSSTAWSDYDRRFVVTADVHQAN TSTWSIPSAPGLDVQLPSDVTDSHWSIPSASGFEVRTPSVQDLTAECAKPRGLAEIMQD FNTAPFQFLSDYRPVPAPRRRPIPSPRSTASAPPVPKPRRTKYQQPPGVARAISEAELD EYIRQHSN" misc_feature 2268..2876 /label=HVD misc_feature 2268..2510 /label=Fragment A misc_feature 2511..2720 /label=Fragment B misc_feature 2664..2699 /label=G3BP binding motif (hypothesized) misc_feature 2721..2873 /label=Fragment C misc_feature 2808..2858 /label=FXR binding motif primer_bind complement(2848..2896) /label=p61-1193 primer_bind 2877..2901 /label=p61-1154 polyA_signal 3006..3127 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3134..3589) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3616..3720 /gene="bla" /label=AmpR promoter promoter 3722..4079 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3930..4065 /label=SV40 ori /note="SV40 origin of replication" CDS 4114..4908 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5140..5187 /label=HSV TK poly(A) signal /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 5516..6104 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" enhancer join(6277,1..303) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 481 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 541 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1141 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg 1201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1261 tgtacaagtc cggactcaga tctcgagctc aagcttccgc acccgcctat agagttatcc 1321 gcggcgatat ctctaagagt gcagaccagg ccatcgtcaa cgccgctaat tccaagggac 1381 agcctggctc tggtgtgtgt ggcgctctct ataggaagtg gcccgctgct ttcgaccggc 1441 agcctatcgc agtgggaaca gcccgactgg tcaaacatga accactgatc atccacgccg 1501 tggggcccaa cttctctaaa atgcctgagc ctgaaggcga tctcaagctc gctgctgcat 1561 acatgagtat tgccagcatt gttaatgctg aaagaatcac aaaaatatcc gtccctctgc 1621 tgagtaccgg aatttactct ggaggtaagg acagggtgat gcagagcctg caccacctgt 1681 ttaccgcgtt tgacacgacc gacgccgacg ttacgatcta ttgtttggat aaacagtggg 1741 aaacaaggat tatcgaagct atacacagga aggagtcagt ggagattctg gatgatgaca 1801 agcctgtgga catagacctg gttcgagttc atcccaattc aagcctggct ggccgccctg 1861 gatactctgt caatgagggg aagttgtata gctatttgga gggtacgcgc ttccatcaga 1921 cagctaaaga tatcgctgaa atccatgcta tgtggcctaa caagtctgaa gcaaatgagc 1981 agatctgtct ctatattctg ggcgaaagta tgagtagcat cagatccaaa tgtcctgtcg 2041 aagaatctga agcctctgca cccccccata ctctcccatg cttgtgtaat tacgcaatga 2101 ccgccgaacg cgtatacaga ttgaggagtg ctaagaaaga gcaatttgct gtgtgttctt 2161 cttttctgct tcccaaatac aggattaccg gcgtgcagaa gttgcagtgt tcaaaacctg 2221 tgctcttttc aggagtcgtg cctccagccg tgcatccaag gaaatacgcc gaaatcatcc 2281 ttgagacacc ccccccaccg gctaccacta cagtgatatg cgagccaaca gttccggagc 2341 gcattccaag ccccgtgatt agccgcgctc catcagctga atcactgctt tccctgggcg 2401 gggtgtcttt ttcaagcagc gccacaagga gtagcacggc gtggtctgat tacgatcgga 2461 ggtttgtcgt gacagcggat gttcatcagg ctaacacctc tacttggagt atccctagcg 2521 cccctggact tgatgtacag ctgccaagcg acgtgacgga cagccactgg agtataccga 2581 gtgcttcagg atttgaagtc cgaacaccgt ctgtccaaga tctgacagcc gaatgtgcca 2641 aacccagagg tctcgctgaa atcatgcaag atttcaacac ggcccctttc cagttcctca 2701 gtgattatag acccgtgcca gcacctagac ggaggccaat cccctcacca agatcaactg 2761 cttccgcacc tcctgtcccg aaaccaagac gcaccaagta tcagcagcca cccggggtcg 2821 ctagggctat tagtgaggcc gagctcgacg agtacataag acaacactca aactgaggat 2881 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg 2941 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 3001 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3121 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 3181 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 3241 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 3301 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 3361 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 3421 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 3481 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 3541 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 3601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3721 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 3961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 4081 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 4141 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 4201 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 4261 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 4321 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 4381 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 4441 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4501 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4561 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4621 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4681 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4741 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4801 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4861 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 4921 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 4981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 5041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 5101 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 5161 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 5221 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 5281 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 5341 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 5401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 5461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 5521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5641 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 5701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 6001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 6061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 6121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 6181 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt 6241 acggggtcat tagttcatag cccatatatg gagttcc //
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